Affiliation:
1. Nagoya Institute of Technology, Gokiso cho, Showa ku, Nagoya 466-8555, Japan
2. ORTHOREBIRTH Co. Ltd, 15-3-303 Chigasaki-Chuo, Tsuzuki-ku, Yokohama 224-0032, Japan
Abstract
In our previous study, we investigated the synergetic effects of inorganic ions, such as silicate, Mg
2+
and Ca
2+
ions on the osteoblast-like cell behaviour. Mg
2+
ions play an important role in cell adhesion. In the present study, we designed a new composite that releases a high concentration of Mg
2+
ions during the early stage of the bone-forming process, and silicate and Ca
2+
ions continuously throughout this process. Here, 40SiO
2
–40MgO–20Na
2
O glass (G) with high solubility and vaterite-based calcium carbonate (V) were selected as the source of silicate and Mg
2+
and Ca
2+
ions, respectively. These particles were mixed with poly(lactic-
co
-glycolic acid) (PLGA) using a kneading method at 110°C to prepare the composite (G-V/PLGA, G/V/PLGA = 4/56/40 (in weight ratio)). Most of the Mg
2+
ions were released within 3 days of immersion at an important stage for cell adhesion, and silicate and Ca
2+
ions were released continuously at rates of 70–80 and 180 ppm d
−1
, respectively, throughout the experiment (until day 7). Mouse-derived osteoblast-like MC3T3-E1 proliferated more vigorously on G-V/PLGA in comparison with V-containing PLGA without G particles; it is possible to control the ion-release behaviour by incorporating a small amount of glass particles.
Funder
KAKENHI
New Energy and Industrial Technology Development Organization
Cited by
9 articles.
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