The relation between “density” of sperm-suspension and fertility as determined by artificial insemination of rabbits

Abstract

Experiments on the physiology of the spermatozoon are in progress in which artificial insemination is used as a test of fertility. One of the variables which enters into the technique is the number of spermatozoa introduced into the vagina of the female. This number will depend upon the “density” of the suspension (i. e., the number of spermatozoa per cubic centimetre) and the volume. The following experiments were undertaken to test the effect of varying the density of the suspension, the volume being constant. Apart from solving a problem of technique it was hoped to gain information on some of the factors which affect fertility or sterility under normal breeding, since it is conceivable that here also the number or density of the ejaculated spermatozoa may play a part.