Assessing the role of family level variation and heat shock gene expression in the thermal stress response of the mosquito Aedes aegypti

Author:

Ware-Gilmore Fhallon12,Novelo Mario12,Sgrò Carla M.3ORCID,Hall Matthew D.3ORCID,McGraw Elizabeth A.124ORCID

Affiliation:

1. Department of Entomology, The Pennsylvania State University, University Park, PA 16802, USA

2. The Center for Infectious Disease Dynamics, The Pennsylvania State University, University Park, PA 16802, USA

3. School of Biological Sciences, Monash University, Melbourne, Victoria 3800, Australia

4. Department of Biology, The Pennsylvania State University, University Park, PA 16802, USA

Abstract

The geographical range of the mosquito vector for many human disease-causing viruses, Aedes aegypti , is expanding, in part owing to changing climate. The capacity of this species to adapt to thermal stress will affect its future distributions. It is unclear how much heritable genetic variation may affect the upper thermal limits of mosquito populations over the long term. Nor are the genetic pathways that confer thermal tolerance fully understood. In the short term, cells induce a plastic, protective response known as 'heat shock'. Using a physiological ‘knockdown’ assay, we investigated mosquito thermal tolerance to characterize the genetic architecture of the trait. While families representing the extreme ends of the distribution for knockdown time differed from one another, the trait exhibited low but non-zero broad-sense heritability. We then explored whether families representing thermal performance extremes differed in their heat shock response by measuring gene expression of heat shock protein-encoding genes Hsp 26, Hsp83 and Hsp70. Contrary to prediction, the families with higher thermal tolerance demonstrated less Hsp expression. This pattern may indicate that other mechanisms of heat tolerance, rather than heat shock, may underpin the stress response, and the costly production of HSPs may instead signal poor adaptation. This article is part of the theme issue ‘Infectious disease ecology and evolution in a changing world’.

Publisher

The Royal Society

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology

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