Simplifying the OpenFlexure microscope software with the web of things

Author:

Collins Joel T.1ORCID,Knapper Joe1ORCID,McDermott Samuel J.2ORCID,Ayazi Filip2ORCID,Bumke Kaspar E.1ORCID,Stirling Julian1ORCID,Bowman Richard W.1ORCID

Affiliation:

1. Centre for Photonics and Photonic Materials, Department of Physics, University of Bath, Bath, UK

2. Cavendish Laboratory, University of Cambridge, Cambridge, UK

Abstract

We present the OpenFlexure Microscope software stack which provides computer control of our open source motorised microscope. Our diverse community of users needs both graphical and script-based interfaces. We split the control code into client and server applications interfaced via a web API conforming to the W3C Web of Things standard. A graphical interface is viewed either in a web browser or in our cross-platform Electron application, and gives basic interactive control including common operations such as Z stack acquisition and tiled scanning. Automated control is possible from Python and M atlab , or any language that supports HTTP requests. Network control makes the software stack more robust, allows multiple microscopes to be controlled by one computer, and facilitates sharing of equipment. Graphical and script-based clients can run simultaneously, making it easier to monitor ongoing experiments. We have included an extension mechanism to add functionality, for example controlling additional hardware components or adding automation routines. Using a Web of Things approach has resulted in a user-friendly and extremely versatile software control solution for the OpenFlexure Microscope, and we believe this approach could be generalized in the future to make automated experiments involving several instruments much easier to implement.

Funder

Royal Society

EPSRC

Publisher

The Royal Society

Subject

Multidisciplinary

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