Affiliation:
1. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt
Abstract
In this study, we have developed two facile spectroscopic methods for quantifying oxybutynin (OBT) hydrochloride in its pure form and tablets using design of experiments (DOEs). The spectroscopic methods depended on the ion-pair complex formation between the tertiary amino group in the drug and eosin in 0.2 M acetate buffer of pH 4. Method I involves spectrophotometric measurement of the absorbance of the developed complex at 550 nm and showed linearity through 1.0–10.0 µg ml
−1
. Method II involves spectrofluorometric measurement of the quenching influence of OBT on the native fluorescence of eosin (λ excitation/λ emission of 304/548 nm) and showed linearity through 1.0–6.0 µg ml
−1
. Critical parameters were identified through preliminary trials and optimized using the DOE. Additionally, the quenching mechanism was investigated and the pathway of the reaction was postulated. The fluorescence quenching constant and thermodynamic parameters were explored using the Stern–Volmer plot and Van't Hoff graph, respectively. Assessments conducted via analytical ecoscale revealed the ‘excellent-greenness’ of the methodology. The two methods have the potentials of being green and fast compared with other reported methods.
Reference41 articles.
1. Overactive Bladder Syndrome: Evaluation and Management
2. British Pharmacopoeia. 2003 London UK: Her Majesty's Stationery Office; 2009. Electronic version.
3. Dwyer J, Tafuri SM, LaGrange CA. 2021 Oxybutynin. In StatPearls [Internet]. Treasure Island, FL: StatPearls Publishing.
4. Spectrophotometric determination of oxybutynin hydrochloride by ion-pair extraction in pharmaceutical preparations;ElSheikh R;Int. J. Pharm. Sci.,2015
5. Determination of Oxybutynin in Pharmaceuticals via Reaction with Mixed Acids Anhydrides: Application to Content Uniformity Testing
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献