EFFECTS OF GROWTH REGULATOR ON INVITRO PROPAGATION OF LILIUM USING BULB SCALE

Abstract

Lilium spp. is the most significant decorative plant and has high demand in the floriculture market. We studied the impact of different growth regulators on culture initiation, shoot proliferation, and root formation of Lilium for disease-free plants from in vitro propagation. The explant basal scale was used for culture initiation, and varying concentrations of BAP (6-Benzylaminopurine) alone and in combination with IAA (Indole-3-acetic acid) showed different responses. It was observed that MS media supplemented with BAP at 1.5 mg/l and IAA at 1.0 mg/l exhibited the maximum number of shoots, i.e., 4.3 shoots and 7 cm length of shoots, which were attained in four weeks. Several concentrations of BAP and Kinetin (KIN) supplemented with MS medium alone or in combination with BAP with IAA, IBA, and NAA were used for shoot proliferation. The results revealed that all treatments resulted in 100% shoot development, with significant variability in the number of shoots and shoot length. MS supplemented with 1.5 mg BAP and 1.0 mg KIN showed the maximum 7.4 number of shoots, with an average shoot length of 7.7 cm. The maximum 11 roots observed in MS supplemented with 1.5mg IBA and the longest roots 4.4cm found in MS with 2mg IAA, and the rooted plantlets were hardened in peat moss media, had the highest ex-vitro survival rate. These results highlight the significance of optimized combinations of growth regulators for improving micropropagation efficiency in the horticulture industry.