Author:
Aslam Javiria,Javed Muhammad,Latif Fariha,Kousar Safina,Iqbal Rehana
Abstract
Present research work was conducted to measure the effects of tertiary metals mixture (Fe+Zn+Mn) on superoxide dismutase (SOD) activity in various tissues of two major carps, Cirrhina mrigala and Labeo rohita at controlled laboratory conditions. 90-day-old fingerlings of both fish species were exposed to 1/4th and 1/5th of their respective 96-hr LC50 value of Fe+Zn+Mn mixture, for 24 days. After 6, 12, 18, and 24-day exposure, fish from each treatment will be sampled, dissected and their tissues viz. brain, gills, kidney, and heart isolated for the SOD enzyme assay. The physical and chemical parameters of test media viz. pH, temperature, dissolved oxygen, total hardness, carbon dioxide, total ammonia, magnesium, and calcium were determined on a 12-hourly basis. It was observed that with an increase in metal concentration, the activity of enzymes increased significantly in both fish species which was maximum at 1/4th of LC50 with the mean value of 49.35±10.04 UmL-1 in C. mrigala. In Labeo rohita, SOD activity decreased with an increase in exposure duration. SOD activity was maximum on day 6 at 52.22±12.91 UmL-1, and on day 24, it was minimum at 35.01±6.91 UmL-1. Among the organs, the SOD activity followed the trend: gills > heart > kidney > brain. The various tissues of metals mixture treated fish Cirrhina mrigala showed significantly increased activity of SOD in comparison to Labeo rohita. All the physicochemical parameters varied significantly at p<0.05 during this study period.
Publisher
Pakistan Journal of Biotechnology - PJBT
Subject
Applied Microbiology and Biotechnology,Biotechnology
Reference43 articles.
1. A.P.H.A. (American Public Health Association). Standard methods for the examination of water and wastewater. (22nd Ed.) American Public Health Association, New York, p. 1360. (2012)
2. Abdullah, S., M. Javed and A. Javid. Studies on acute toxicity of metals to the Fish (Labeo rohita). Int. J. Agric. Bio., 2: 333-337. (2007)
3. Avci, A., M. M. Kacmaz and I. Durak. Peroxidation in muscles and liver tissues from fish in a contaminated river due to a petrolium refinery industry. Ecotoxicol. Environ. Saf., 6:101-105. (2005)
4. Azmat, H., M. Javed and G. Jabeen. Acute toxicity of aluminium to the fish (Catla catla, Labeo rohita and Cirrhina mrigala). Pak. Vet. J., 32: 85-87. 2012 Barata, C., D. J. Baird, A. J. A. Nogueira, A. M. V. M. Soares and M. C. Riva. Toxicity of binary mixtures of metals and pyrethroid insecticides to Daphnia magna Straus. Implications for multi-substance risks assessment. Aquat. Toxicol., 78: 1-14. (2006)
5. Batool, M., S. Abdullah and K. Abbas. Antioxidant enzymes activity during acute toxicity of chromium and cadmium to Channa marulius and Wallago attu. Pak. J. Agri. Sci., 51: 1017-1023. (2014)