Affiliation:
1. Aydın State Hospital
2. ADNAN MENDERES UNIVERSITY
3. AYDIN ADNAN MENDERES UNIVERSITY
Abstract
Objectives: To investigate the role of blood brain barrier biomarkers for the detection of experimental cerebral ischemia in rats.
Methods: Forty adult male Wistar albino rats with a mean age of 4–6 months and an average weight of 350–400 g were used in the study. The rats were divided into five ischemia groups (control, 1.5 h of ischemia, 4.5 h of ischemia, 6 h of ischemia, and 24 h of ischemia). Cerebral ischemia was achieved by unilateral ligating of CCA and ECA at the same time. After surgical preparation and awaiting for appropriate ischemia time we collected blood and brain tissue samples. Then we investigated serum occludin, claudin-5 and JAM-A levels from blood samples and the apoptotic index and percentages of pycnotic nucleus from brain tissues histologically. The obtained data were analyzed using IBM SPSS Statistics software package version 18 and the Jamovi software package.
Results: Serum JAM-A level showed a statistically significant difference in all ischemia groups compared with the control group (p<0.05). Serum claudin-5 level, a statistically significant difference was found between the control group and the 6-h ischemia group (p<0.05), while no significant inter-group differences were determined for the serum occludin level. As a result, in our experimental focal cerebral ischemia model, serum JAM-A showed more significant and rapid increases compared to occluding and claudin-5. (Since four rats of the 24-h ischemia group died before completing their term, these group data were not statistically evaluated.)
Conclusions: Serum JAM-A might be successfully used in the early stages of ischemic stroke. The presence of hemiparesis or hemiplegic effects in all rat ischemia groups and the significant increases in pyknotic and apoptotic cell counts histologically suggest that our model is successful for focal cerebral ischemia.
Funder
The Committee for Scientific Researches of Adnan Menderes University
Publisher
Adnan Menderes University
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