Influence of Graphene Oxide-Doped Bio-Gelatin Material on Hemodynamics of Sepsis

Abstract

To obtain astragalus polysaccharide (APS)-magnetic graphene oxide (MGO) nanoparticles (NPs), MGO was prepared by chemical co-precipitation, and then loaded with APS NPs. After the physical characterization of the material was analyzed, it was adopted to treat patients with sepsis injury to study its mechanism. Then, changes in cytokine levels in the patient’s myocardial tissue were analyzed. Giving that septic myocardial injury-related neutrophil gelatinase (neutrophil gelatinase-associated lipocalin (NGAL) and cystatin) was related to hemodynamics, changes in the patient’s hemodynamic indicators were analyzed before and after the addition of nanomaterials. Moreover, changes of the patient’s gelatinase markers before and after the preparation of nanomaterials were also analyzed. The results showed that the Fe3O4 magnetic NPs were coated on the surface of GO while successfully adsorbing APS. The sample recovery rate was more than 100%, and the relative standard deviation was 0.48%. The in vitro release showed that the NPs were easily released in an acidic environment. Clinical trials showed that the levels of pro-inflammatory cytokines (tumor necrosis factor (TNF)-α, interleukin (IL)-β, etc.) in myocardial tissue were dramatically decreased (P < 0.05), while the levels of anti-inflammatory cytokines (IL-2, IL-4, etc.) were greatly increased (P < 0.05). The NGAL level at the arterial and venous ends both decreased remarkably (P < 0.05). The total mass transfer ratio (Mtr) increased obviously while the mass adsorption rate (Mad) decreased significantly (P < 0.05). In addition, hemodynamic indicators such as cardiac output (CO) and diastolic arterial pressure (DAP) all improved notably.