Preparation of Dopamine Nanoparticles and Its Application in the Treatment of Neonatal Scleredema

Abstract

The polydopamine (PDA) nanoparticles were prepared by the aqueous phase oxidation, and doxorubicin (DOX) was applied on surface of PDA under appropriate temperature and Pondus Hydrogenii (pH) conditions. The human umbilical vein-derived mesenchymal stem cells (hUV-MSCs) were sub-cultured, and then destroyed by lysing and freeze-thaw to obtain the stem cell membrane (SSM) fragments, which were squeezed using the polycarbonate porous membrane and then wrapped in the surfaces of the PDA nanoparticles by means of water bath ultrasound. In this way, the biomimetic nano medicine was synthesized. In the experiment, the DOX release amount of the nanomedicine under different conditions was analyzed. Under the precondition that the concentration of the drug was changed, and it was used to explore proliferation activity of human esophageal cancer cell (Eca-109). Mice with sclerosed skin caused by scleredema were selected as the test subjects to observe the inhibitory effect of the prepared drugs on its fibrosis. In addition, 90 cases of neonatal scleredema were selected and grouped to verify the improvement effect of nano-medicine injection on their symptoms. The results relaved that average particle size (APS) of synthesized drug was 77.6±8.5 nm, and the drug loading of DOX loaded on the surface of PDA was 18.4%. The transmission electron microscopy (TEM) displayed that surface of prepared drug was structured with cell membrane, and the release of DOX was correlated with the pH of the drug solution. Under the condition of a certain DOX concentration, the prepared nano-drug could kill the Eca-109 cells strongly (P < 0.05). In the sclerosing phase, the skin thickness and collagen index of the mice could be reduced by injecting different doses of the drug solutions (P < 0.05), while the content of transforming growth factor β (TGF-β)/platelet-derived growth factor A-chain (PDGFA) was decreased (P < 0.05). The severity score of newborn children was greatly reduced after drug injection treatment; and the temperature recovery time, sclerosis disappearance time, and hospitalization time were shortened to a great extent (P < 0.05).