Nanoparticle-Encapsulated miR-27-Mediated Wnt/β-Catenin Signaling Pathway Inhibits Cervical Cancer

Abstract

Non-viral carrier nanomaterials are commonly used for molecular targeted therapy. This study explored the effects of miR-27 on cervical cancer using nanoparticles as a carrier. Hela cells were used to build cervical cancer tumor model. 20 nude mice were randomly assigned into control group, empty vector group (treated with glucopyranosyl lipid adjuvant-human serum albumin (GLA-HSA), miR-27 group (treated with GLA-HSAencapsulated miR-27), and 17-AAG group (treated with GLA-HSA-encapsulated miR-27 and 17-AAG mixed culture medium), followed by analysis of tumor morphology, apoptosis and expression of Wnt/β-catenin and apoptosis factors (Bcl-2, caspase-3). The size of transplanted tumor was smallest in 17-AAG group on 7th day, followed by miR-27 group (P < 0.05) compared to control group and empty carrier group without different between them (P > 0.05). The miR-27 group and 17-AAG group had highest tumor inhibition rate and apoptotic cell number and apoptosis index compared to other two groups without difference between them (P > 0.05). miR-27 group and 17-AAG group showed lowest expression of CA125, CEA, Wnt, β-catenin, Bcl-2, and caspase-3 with lower level in 17-AAG group than miR-27 group (P > 0.05). Loading miR-27 small molecule substance inhibited cervical cancer tumor growth and promoted tumor cell apoptosis with decreased CA125 and CEA expression, indicating that the miR-27 coated with nanoparticles may inhibit Wnt/β-catenin signal pathway, thereby up-regulating Bcl-2 and caspase-3, and finally exerting an anti-tumor effect.