Preparation of Nanoparticles and Their Application for Evaluating the Position of Fetal Medullary Cone Combine with Ultrasound

Abstract

This study was to analyze the application of liquid Perfluorohexane (PFH) nanoparticles (NPs) combined with ultrasound image in the diagnosis of the location of the fetal medullary cone (MC). The rotary evaporationultrasound (REUS) method was adopted to prepare the targeted phase-shift liquid PFH NPs carrying 10-Hydroxy camptothecin (HCPT) (TPS-HCPT-PFH-NPs), which were applied in ultrasound detection to locate the MC of 126 fetuses. The results showed that under 4 °C, TPS-HCPT-PFH-NPs showed a slow upward trend with time, but the increase was not significant; the particle size of TPS-HCPT-PFH-NPs showed an obvious increasing trend at the 0th–40th hour, and the growth rate was slow after the 40th hour. Its final drug release rate (DRR) reached 78.22%. Fetal MC distributed in vertebral body L2–L3 in the most cases (51 cases), followed by vertebral body L2 (32 cases), L3 (23 cases), and L1–L2 (17 cases). The number of caudal vertebral bodies in fetuses >35 weeks was obviously more than that of fetuses at 20–25 weeks and 25–35 weeks (P < 0.05). The MC position of fetuses at >35 weeks was observable greater than that of fetuses at 20–25 weeks and 25– 35 weeks (P < 0.05). The contrast-enhanced ultrasound based on TPS-HCPT-PFH-NPs showed visibly higher diagnostic accuracy and shorter running time in contrast to the routine ultrasound (P < 0.05). In short, the TPSHCPT-PFH-NPs showed good stability, thermal phase-shift, and DRR. Thus, it could effectively improve the diagnostic effect of ultrasound, and could be promoted in clinic. The location of fetal MC was mainly distributed in vertebra L2–L3, which showed a reliable linear correlation with gestational age.