Biological Activity Test of Dendrobium Huoshanense and Its Application in the Detection of Environmental Pollutant Phenols

Author:

Wang Yujuan1,Dai Jun2,Wei Peipei3,Cai Yongping4,Han Bangxing1

Affiliation:

1. Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, West Anhui University 237012, Anhui Province, China

2. Key Laboratory of Biomimetic Sensor and Detecting Technology of Anhui Province, West Anhui University 237012, Anhui Province, China

3. College of Biological and Pharmaceutical Engineering, West Anhui University 237012, Anhui Province, China

4. Yongping Cai, Anhui Agricultural University, Hefei 230036, China

Abstract

ABSTRACTIn this research, a new type of carbon quantum dot was prepared from Dendrobium huoshanense via one-step hydrothermal reaction at 200 °C for 6 h. The as-derived carbon quantum dots exhibited good fluorescence properties, with quantum yield of 23.57%. The extraction and determination of Dendrobium huoshanense enzymes activity were performed for different incubation times to study the theoretical reference for the dynamic changes of the main enzyme activity in the process of Dendrobium huoshanense growth and the solid processing industry. The results demonstrated that Dendrobium huoshanense was a low toxic carbon source material. Moreover, the prepared carbon quantum dots exhibited high sensitivity for the detection of nonylphenol, allowing a range of linear response of 0.5–50 µM (R2 = 0.9997). The detection limit was a slow as 95.32 nM. These results indicated that fluorescence internal filtration effect influenced nonylphenol-induced quenching of the Dendrobium huoshanense-carbon quantum dots fluorescence. The Dendrobium huoshanense-carbon quantum dots were successfully used to track nonylphenol in environmental samples. Therefore, their exploitation offers a promising approach for environmental pollutant detection.

Publisher

American Scientific Publishers

Subject

General Materials Science

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