Preparation of Metformin/Doxorubicin-Bovine Serum Albumin-Hyaluronic Acid-Carbon Dots Nanoparticles and Their Application in Treating Polycystic Ovary Syndrome on Matrix Metalloproteinase-9 and Vascular Endothelial Growth Factor of Patient Combine with Metformin

Author:

Cheng Lixia1,Jiang Guanyan2,Yu Hongchao3,Liu Shuohui2

Affiliation:

1. Department of Endocrinology, Weifang People’s Hospital, Weifang, 261041, Shandong, China

2. Department of Outpatient, Weifang People’s Hospital, Weifang, 261041, Shandong, China

3. Weifang Medical University, Weifang, 261053, Shandong, China

Abstract

At present, carbon dot nanoparticle has good light stability and bioavailability, which has become a novel type of nano material widely employed in medicine. This investigation aimed to study the effects of nanomedicine based on Metformin/doxorubicin-bovine serum albumin-hyaluronic acid-carbon dots (Met/DOX-BSA-HA-CD) on matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) of polycystic ovarian syndrome (PCOS) patients. First, Met/DOX-BSA-HA-CD was prepared from Met and doxorubicin (DOX) and BSA-HACD solution. 30 PCOS patients accepted by hospital were deemed as experimental group, and 30 healthy married women were taken as controls. Met/DOX-BSA-HA-CD nanomedicine was prepared and applied to the treatment of 30 PCOS patients. The results showed that the average particle size of Met/DOX-BSA-HA-CD was about 120 nm. MMP-9 immunohistochemical H-Score (285.32±36.41 points) of the endometrial glandular epithelium of experimental group was considerably inferior to controls (364.35±26.74 points) (P < 0.05). VEGF level of experimental group (103.25±20.64 pg/mL) was substantially superior to controls (86.32±14.21 pg/mL) (P < 0.05). Moreover, serum VEGF level was positively correlated with body mass index (r = 0.236, P < 0.05), and negatively correlated with follicle stimulating hormone/luteinizing hormone (FSH/LH) (r =−0.061, P < 0.05). The carbon dots prepared in this study had good light stability. The ratio of nanocarrier to drug: bovine serum albumin-hyaluronic acid-carbon dots:metformin = 4:1 was determined as the feeding ratio, and the embedding rate was 39.8%. The expression of MMP-9 in the endometrial glandular epithelium of PCOS patients was expected to be an indicator of endometrial receptivity. PCOS patients had endocrine disorders and abnormal VEGF levels.

Publisher

American Scientific Publishers

Subject

General Materials Science

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