Affiliation:
1. Department of Translational Medicine Center, Xi’an Chest Hospital, Shaanxi 710061, China
2. Department of Laboratory Medicine, Xi’an Chest Hospital, Shaanxi 710061, China
3. Xi’an Mental Health Center, Chang’an District, Xi’an, Shaanxi 710061, China
Abstract
Differential expression of miR-223 is related to the progression of tuberculosis. This study mainly explored the effect of albumin nanoparticles carrying miR-223 on macrophage autophagy in tuberculosis and related mechanisms. Sixty SD rats were selected and randomly divided into (n
= 10 for each, 12 mg/mL) sham group, model group, miR-223 group, isoniazid group, STAT3 pathway inhibitor group, and STAT3 pathway agonist group. The rat body weight and number of Mycobacterium tuberculosis were measured and expressions of miR-223, p-STAT3, STAT3, Beclin1, and LC3-II/LC3-I
were determined. Moreover, the targeting relationship between miR-223 and STAT3 was analyzed. The difference in the rat weight between four groups was not significance in the 1st day (P > 0.05), while the weight of the model group was lowest on the 15th day, and weights of the other
three groups increased significantly (P < 0.05). The number of Mycobacterium tuberculosis in the model group reached (8.58±0.22) lg10, while the number of Mycobacterium tuberculosis in the isoniazid group and miR-223 group were lower than the model group (P
< 0.05). The expression of miR-223 in the model group was lower than in the other groups (P < 0.05). The expressions of p-STAT3 and STAT3 were highest but the expressions of Beclin1 and LC3-II/LC3-I were lowest in the model group (P < 0.05). The expressions of p-STAT3
and STAT3 were highest in the model and STAT3 pathway agonist groups, while expressions of Beclin1 and LC3-II/LC3-I, and the number of Mycobacterium tuberculosis were lowest, while the sham and STAT3 pathway inhibitor groups showed opposite trends (P < 0.05). Moreover, significant
difference was found in comparisons between model group and STAT3 pathway agonist group (P < 0.05), but no such significance existed between the sham and STAT3 pathway inhibitor groups (P > 0.05). In conclusion, albumin nanoparticles-encapsulated miR-223 slowed release
of miR-223 to the body, which directly inhibited the activity of STAT3 pathway and downregulated the expression of STAT3 and p-STAT3, but up-regulated the expression of Beclin1 and LC3-II/LC3-I expression, ultimately promoting macrophage autophagy in tuberculosis. The main mechanism for miR-223
was related to inhibition of the STAT3 pathway.
Publisher
American Scientific Publishers
Subject
General Materials Science