Preparation of Targeted Nanoparticles and Activity of Anti-Helicobacter Pylori

Abstract

Helicobacter pylori (Hp) is a gram-negative spiral bacterium that grows in a microaerobic environment and can be found in people with gastritis and ulcer diseases. First, the drug 5-aminosalicylic acid (5-ASA) for the treatment of ulcerative colitis (UC) was investigated in this study. In order to increase the drug concentration in the diseased colon, silica nanoparticles (SNP) were prepared by the microemulsion method. Then, the drug 5-ASA was grafted onto the surface of the modified SNP, so as to obtain the targeted nanoparticles 5-ASA-SNP. The synthesis and drug loading of the materials were analyzed, and then, they were used for the treatment of colitis in the experimental mice. The inhibition of Hp ATCC700392 strain was explored according to the material selection. In the experiment, the synthesized targeted nanoparticles (5-ASA-SNP) had uniform particle size, smooth surface, and good dispersibility. Besides, the drug loading of 5-ASA reached 13.75±2.6%. In the biological toxicity analysis, the survival rate of Caco-2 cells in the 5-ASA-SNP material configuration solution reached 83.65±1.86%. In the group testing of the disease activity index (DAI) and colonic histopathology score, the H-5-ASA group and 5-ASA-SNP group were lower sharply than N-5-ASA group (P < 0.05). The expression of IL (interleukin)-6 and tumor necrosis factor (TNF)-a protein in the serum of the grouped mice from the H-5-ASA group and 5-ASA-SNP group were lower steeply than the expression of the N-5-ASA group (P < 0.05). The agar double dilution method was adopted to prepare the Hp solution according to different materials. The results confirmed that 5-ASA-SNP solution had the best antibacterial effect, and increasing the concentration of the solution can greatly inhibit the activity of Hp (P < 0.05).