Clinical Study of the Treatment of Klebsiella pneumoniae with Comprehensive Nursing Intervention Combined with New Nano Silver

Abstract

To investigate the inhibitory effect of new nano silver (nAg-NPs) on Klebsiella pneumoniae producing extended spectrum β-lactamases (EBLs). Clinical interventions are mainly directed to inpatients, or patients with obvious discomfort, symptoms, and signs needing outpatient examination, referral, and clear diagnosis. We randomly selected 88 patients from the rehabilitation department of our hospital from November 2017 to June 2019, and divided them into observation and control groups by drawing lots. Taking ESBL K. pneumoniae as the research object, the bactericidal effect of nAg-NPs was determined using the coating method; the minimal inhibitory concentration (MIC) was measured using the broth microdilution method, and the mechanism of action of nAg-NPs on ESBL K. pneumoniae was evaluated by electron microscopy. After the implementation of different nursing management modes, the incidence of risk events in the observation group was significantly lower than that in the control group, and nursing satisfaction was significantly higher than that in the control group. nAg-NPs (≥0.5 g/mL) had 100% bactericidal effect on ESBL K. pneumoniae, 0.05 g/mL nAg-NPs had obvious bactericidal effect on ESBL K. pneumoniae, 5 g/mL nAg-NPs had obvious bactericidal effect on ESBL K. pneumoniae for 5, 15, 30 and 60 min. Additionally, nAg-NPs showed 3.12 g/mL of MIC. Furthermore, nAg-NPs had a significant effect on the morphology of K. pneumoniae. nAg-NPs shows obvious inhibitory effect on ESBL K. pneumoniae. These results will provide an experimental basis for the further study and clinical application of nAg-NPs with the help of clinical nurses.