In Vitro Anti-Coagulant Effects of Fucoidan Sulfate Grafted on TiO2 Nano-Film

Abstract

The objective of this study was to examine anticoagulant effects of fucoidan sulfate grafted on a TiO2 nano-film. Using silica sol as a film-forming binding agent and high-molecular-weight polyvinyl alcohol as a film-forming aid, a nano-TiO2 film was produced by applying a bonding film-forming method. Photocatalytic performance and resistance to heat, corrosion, and aging were investigated. Using glutaraldehyde as a cross-linking agent, brown algal polysaccharide sulfate was grafted onto the surface of NH3 plasma-treated nano-film. The effects of pH, reaction temperature, and reaction time on grafting effects of nano-film are discussed. XPS was used to analyze chemical elements on the surface of nano-film, and partial thromboplastin time (APTT), thromboplastin time (PT), and thrombin time (TT) were used to evaluate anticoagulant activity of the graft nano-film In Vitro. The TiO2 nano-film showed excellent resistance to heat, water, corrosion, and aging, and produced good interfacial adhesion performance. The grafting process optimum occurred at pH 2, 40 °C, and a reaction time of 6 h. Under these conditions, the optimal value of grafted silk fibroin membrane APTT was 72 s, and coagulation time of APTT and TT was increased by 37 s and 6 s, respectively, compared with the control, suggesting anticoagulant effects. Regarding stability, grafted fucoidan sulfate did not dissolve easily from the matrix; therefore, this product appears to be a promising novel biomaterial with effective anticoagulant properties.