Affiliation:
1. School of Life Sciences, University of Kwa-Zulu-Natal, Durban 4000, South Africa
2. School of Chemistry and Physics, University of Kwa-Zulu-Natal, Durban 4000, South Africa
Abstract
Hydrotalcites and amino acid functionalized hydrotalcites (aa-HTs) were synthesized using the co-precipitation method. These compounds were characterized using X-ray diffraction (XRD), inductively coupled plasma-optical emission spectroscopy (ICP-OES), Fourier transform infrared spectroscopy
(FTIR), transmission electron microscopy (TEM), and nanoparticle tracking analysis (NTA). The optimal siRNA binding capacity was determined using gel retardation assays. Cytotoxicity studies using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the
siRNA:HT complexes were relatively non-cytotoxic to HeLa-tat-luc cells. Gene silencing studies showed that the aa-HTs elicited significantly higher levels of gene knockdown when compared to the non-functionalized HTs. Overall, these HTs have shown the potential to bind, protect and
efficiently deliver siRNA, in vitro.
Publisher
American Scientific Publishers
Subject
Condensed Matter Physics,General Materials Science,Biomedical Engineering,General Chemistry,Bioengineering
Cited by
6 articles.
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