Affiliation:
1. Department of Interventional Radiology, The Affiliated Hospital of GuiZhou Medical University, Guiyang, Guizhou, 550000, China
Abstract
This study assessed the mechanism of tanshinone A in regulating biological characteristics of Hematopoietic Stem Cell (HSC) in liver cirrhosis through targeting of miR-9a-5p. HSC cells were divided into negative control group and stimulated miR-9a-5p inhibitor group. Transfection was
performed according to specification of the kit. Expression of miR-9a-5p was assessed with Real-time polymerase chain reaction (PCR). Cell proliferation was tested with flow cytometry (FCM), and α-smooth muscle actin (SMA) and Type I collagen expressions were detected with Western
Blot assay. Caspase-3 activity was tested with spectrophotometry, while variation of inflammatory factor was detected with enzyme-linked immunosorbent assay (ELISA). There was higher miR-9a-5p level in HSC induced by Chemokine (C-C motif) ligands 4 (CCL-4). Biological characteristics of HSC
induced by CCL-4 was restrained by down-regulation of miR-9a-5p, and presentation quantity of α-SMA and Type I collagen was reduced. So, occurrence of inflammation and migration of HSC could be restrained. The presentation quantity of Type I collagen was reduced with tanshinone
A, and expression of miR-9a-5p was reduced. HSC characteristics in liver cirrhosis were affected by tanshinone A probably through regulating miR-9a-5p. It could provide a brand-new selection for treatment on liver cirrhosis.
Publisher
American Scientific Publishers
Subject
General Materials Science
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