Gold-enhanced current-volt dielectrode junction for biosensing with an aptamer-insulin-like growth factor-1-antibody sandwich pattern

Author:

Liu Lanbo1,Gopinath Subash C. B.2,Wu Yuan Seng3,Zhao Weichao1

Affiliation:

1. Department of Cardiovascular Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, 051411, China

2. Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000 Kangar, Perlis, Malaysia

3. Centre for Virus and Vaccine Research, School of Medical and Life Sciences, Sunway University, Selangor 47500, Malaysia

Abstract

Insulin-like growth factor-1 (IGF-1) is a hormone comprising seventy amino acids that is a key regulator of muscle and bone health and is highly related to the development of cancer. An aptamer-antibody sandwich assay was performed to determine the presence of IGF1 on a gold nanoparticle-enhanced dielectrode junctional sensing surface by using this surface as a transducer. High-resolution microscopy observations showed the characteristics of the sensing surface and gold nanoparticles. The gold nanoparticle-enhanced surface induces high immobilization of the anti-IGF1 aptamer and enhances the interactions of IGF1. With this probe surface, the aptamer-IGF1-antibody sandwich assay resulted in an increased current for each different solution concentration of IGF1, and the detection limit was 0.5 pg/mL with an R2 value of 0.9631 on a linear calibration curve with IGF1 solution concentrations of 1–16 pg/mL. In addition, IGF1-spiked serum reached a similar limit of detection of 0.5 pg/mL, confirming that selective IGF1 detection occurred for the biological sample.

Publisher

American Scientific Publishers

Subject

General Materials Science

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3