Research on mechanism of vitamin A combined with regenerated oxidation-reducing nanomedicine in treating rats with dry eye

Abstract

The aim of this study was to assess the mechanism of vitamin A combined with regenerated oxidationreducing nanomedicine in treating rats with dry eyes. Sprague Dawley (SD) rats were divided into control set, Dry eye disease (DED) set, VA set, CeO2 NPs set and combined set. The model of DED rat was prepared through injection with Scopolamine Hydrobromide and quantity of tear fluid was detected. The breakup time (BUT) was detected with fluorescein sodium test paper, and degree of corneal injury was evaluated with fluorescein sodium staining method. The change of superoxide dismutase (SOD) activity and reactive oxygen species (ROS) content was analyzed and secretion of IL-6 and IL-1β in serum was tested with enzyme-linked immunosorbent assay (ELISA) method. The protein expressions of Bcl-2 and Bax were analyzed with Western Blot assay and quantity of tear fluid in the treated sets was increased and BUT was lengthened. The corneal injury was improved, and ROS content was reduced and SOD activity increased. The secretion of IL-6 and IL-1β was reduced. Moreover, Bcl-2 expression was increased, while Bax expression was reduced. The action in combined set was more notable and DED was treated by vitamin A combined with regenerated oxidationreducing nanomedicine through improving oxidative stress and restraining the inflammatory secretion. The apoptosis could be regulated.