Mechanism of 5-fluorouracil nanoparticles on releasing skin squamous cell carcinoma through regulation of Wnt/β-catenin expression

Abstract

5-FU nanoparticles were prepared in this study to discuss mechanism of 5-fluorouracil nanoparticles on releasing SSCC through regulating Wnt/β-catenin expression. The characteristics of nanomaterial were analyzed through electron microscope. The releasing characteristics in vitro and in vivo were also detected. Proliferation and apoptosis of SCC cell line in control and 5-FU nanoparticles groups were detected through FCM and MTT method. The tumor volume was recorded and average particle size was 310 nm with uni-form distribution. The apoptosis of MCF-7 cells in the 5-FU nanoparticles group was increased notably, and there were abundant circular floating apoptotic cells. The quantity of active MCF-7 cells was reduced notably and proliferation of SSCC could be restrained by 5-FU nanoparticles through regulation of Wnt/β-catenin expression.