Molecular mechanism of new type of lipid nanoparticles on gastric carcinoma by autophagy and apoptosis of caspase family being targeted with miR-199a-3p

Abstract

This study assesses the molecular mechanism of new type of lipid nanoparticles on gastric carcinoma by autophagy and apoptosis of Caspase family being targeted with miR-199a-3p. Gastric carcinoma tissue was set as observation group while tissue from two centimeters of gastric carcinoma was set as control group. miR-199a-3p and Caspase3 expression was detected with RT-PCR. Proliferative and apoptotic activity was detected with 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and flow cytometry (FCM), while the quantity of invasion and migration were detected with Transwell. The presentation quantity of miR-199a-3p in test group was elevated notably compared with control group. There was a negative regulation between and Caspase 3. The cellular infiltration of gastric carcinoma was notably reduced with Caspase3 nanoparticles being targeted by transfection of miR-199a-3p. Gastric carcinoma growth rate in test group was reduced when miR-199a-3p level was interfered with si-RNA. The apoptosis could be reduced if there was over presentation of miR-199a-3p. Cell invasive and metastatic activity was notably reduced by the Caspase3 nanoparticles being targeted by transfection of si-miR-199a-3p. The invasive and metastatic activity of gastric carcinoma was prompted with increased expression of Caspase3 and reduced miR-199a-3p expression. Cell behaviors were effectively enhanced by directed nanoparticles.