Role of bone marrow mesenchymal stem cell-conditioned media combined with polystyrene nanoparticles in rat osteomyelitis and macrophage polarization

Author:

Pan Deyue1,Ji Renchen1,Yin Peng1,Chen Guang1

Affiliation:

1. Department of Orthopedic Trauma, Second Affiliated Hospital of Dalian Medical University, Dalian, 116000, Liaoning Province, China

Abstract

This study aimed at investigating the potential role of bone marrow mesenchymal stem cell-conditioned media (BMSC-CM) incorporated with polystyrene nanoparticles (PS-NPs) in inflammatory response in osteomyelitis and clarifying its underlying mechanism. BMSCs were collected from 25 osteomyelitis and healthy rats, and their conditioned media (CM) were collected. Enzyme-linked Immunosorbent Assay (ELISA) was used to determine the expression levels of inflammatory factor IL-10, while the BMSCs were identified by surface antigens. After treatment of BMSCs, BMSC-CM, and BMSC-CM with PS-NPs, flow cytometry was conducted to assess apoptosis of inflammatory cells and macrophage polarization-specific protein, and mRNA expressions were detected. The expression level of IL-10 was up-regulated in the osteomyelitis rats. With primary BMSCs in a long spindle shape, the MSCs were positive for CD44 (99.87%) and negative for antigen CD34 (4.32%). The BMSC-CM combined with PS-NPs caused increased macrophage polarization-related genes (BMP-2, MCP-1, iNOS, CD206, Arg-1, TGF-β) with decreased IL-10 expression. The BMSC-CM combined with PSNPs might inhibit the polarization of BMSCs to macrophages and alleviate inflammation in osteomyelitis.

Publisher

American Scientific Publishers

Subject

General Materials Science

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Application and Potential of Nanobiomaterials in Bone Regeneration;Journal of Biomaterials and Tissue Engineering;2024-01-01

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