Multifunctional Biocompatible Rhinacanthus nasutus Based TiO2-Doped CeO Nanoparticles Synthesis and their Biomedical Evaluations; In-Vitro/In-Vivo Approach

Abstract

The work intended to evaluate the potential wound healing properties of TiO2 doped CeO nanoparticles with the assistance of Rhinacanthus nasutus. Furthermore, an assessment was conducted on the nanoparticles to determine their antioxidant, cytotoxic, anti-diabetic, and anti-inflammatory inhibitory properties, as well as their toxicity in albino rats. The nanoparticles were synthesized in the green method and subjected to characterization through various methods including UV-visible spectroscopy, SEM for morphological study, FTIR to identify functional groups, XRD, and for elemental analysis EDAX. The morphology of the observed nanoparticles were predominantly spherical, exhibiting an agglomerated structure. The findings indicated that approximately 49% of the nanoparticles exhibited DPPH antioxidant activity, as determined by an IC50 value of 2.8 g/mL. The nanoparticles exhibited cytotoxicity in the brine shrimp lethality assay when administered at a concentration of 50 g/mL. Additionally, they displayed notable inhibitory activity against-amylase, with an IC50 value of 2.981 g/mL. The Ames test yielded negative results, suggesting that the nanoparticles exhibited non-toxic properties. In general, the study substantiated the prospective biological uses of TiO2-doped CeO nanoparticles.