In Vitro Characterization and Histological Evaluations of Paclitaxel-Loaded Nanofibrous Matrices, as Potential Local Therapeutic for Treating Breast Cancer: An In Vitro and In Vivo Study

Abstract

In the current study, paclitaxel was loaded into electrospun gelatin nanofibers in order to produce a potential anti-cancer membrane. The developed system was studied for its cytotoxic effects against breast cancer MCF-7 cells. Furthermore, biodegradation rate, microstructure, cell migration assay, and tensile strength were performed for the evaluation of anticancer local therapeutic. Study showed that, the electrospun membranes were composed of smooth and web-like nanofibers. In addition, the paclitaxel-loaded nanofibers were toxic against breast cancer cells and prevented their migration. Real time PCR assay showed that the anticancer activity of the developed system might be through regulation of DJ-1/KLF 17 signalling pathway.