The Expression of Circ_0000615 in Tenon’s Capsule Fibroblasts and Its Effect on Cell Proliferation and Migration

Abstract

To explore the expression of circ_0000615 in HTFs and its effect on cell proliferation and migration. With in vitro culture of HTFs, qRT-PCR was performed to detect the expression of circ_0000615 in HTFs. Cells in logarithmic phase were taken for subsequent experiments, and the following groups were constructed, including HTF blank control group (C group); HTFs+10 ng/mL TGF-β1 group (TGF-β1 group); HTFs+si-NC group (si-NC group); and HTFs+si-circ_0000615 group (si-circ_0000615 group). CCK-8 assay was performed to detect cell proliferation, Cell Monoclonal Assay was used to detect Cell Monoclonal Formationand, Transwell assay was conducted simultaneously to detect cell migration. According to the results of qRT-PCR, compared with C group, after induction of HTFs with TGF-β1 for 24 h and 48 h, TGF-β1 group showed significantly increased expressions of circ_0000615, with statistically significant differences (P < 0.05). After induction of HTFs with TGF-β1, compared with C group, TGF-β1 group had enhanced cell proliferation, monoclonal formation and migration, showing statistically significant differences (P < 0.05). Furthermore, after cell transfections for HTFs, compared with si-NC group, si-circ_0000615 group showed obviously downregulated expression of circ_0000615 in HTFs, accompanied by evidently weakened cell proliferation, monoclonal formation and migration, statistically significant differences (P < 0.05). Circ_0000615 is highly expressed in HTFs. A silenced expression of circ_0000615 may inhibit the proliferation and migration of HTFs.