MicroRNA-532 Promotes Proliferation, Metastasis and Autophagy in Esophageal Cancer via Targeting Naked Cuticle 1

Abstract

The aim of this study was to elucidate the role of microRNA-532 in influencing the progression of esophageal cancer and cell autophagy through negatively regulating Naked cuticle 1 (NKD1) level. Relative levels of microRNA-532 and NKD1 in esophageal cancer tissues and corresponding paracancerous ones were detected. The relationship between microRNA-532 level and overall survival in esophageal cancer patients was statistically analyzed. Subsequently, regulatory effects of microRNA-532 and NKD1 on viability, metastasis and autophagy in TE-1 and EC-109 cells were examined. The interaction between microRNA-532 and NKD1 was finally explored. MicroRNA-532 was upregulated in esophageal cancer tissues and its high level indicated poor prognosis in esophageal cancer patients. Serving as the downstream gene binding microRNA-532, NKD1 level was negatively regulated by microRNA-532. Knockdown of microRNA-532 weakened viability and metastasis, as well as downregulated autophagy-associated genes (Atg5, LC-3I and LC-3II) in TE-1 and EC-109 cells. Knockdown of NKD1 achieved the opposite results. MicroRNA-532 promotes proliferation, metastasis and autophagy in esophageal cancer by downregulating NKD1. It is believed that microRNA-532 may be a promising therapeutic target for esophageal cancer.