Biao and Ben Acupoints Combination Regulates Mitochondrial Function Through Purinergic P2X7 Receptor in Rats with Myocardial Ischemia

Abstract

We aimed to investigate the possible mechanisms of electroacupuncture at the Biao and Ben acupoints in improving myocardial ischemia-induced mitochondrial dysfunction. The study examined mitochondrial structure, ATP production, cytosolic calcium accumulation, P2X7 receptor, and mitochondrial-associated oxidative stress factors in rats with a myocardial ischemia model. Eighty-four male and female SD rats were randomly divided into seven groups: Control, Sham, Model, High-dose/Low-dose ZnCl2, electroacupuncture at the Neiguan point, and the Combination of Biao and Ben acupoints. The electroacupuncture groups, including the Neiguan point and Biao and Ben acupoints group, were treated with acupuncture for 10 minutes per day for 21 days. The combination of Biao and Ben acupoints decreased the damage to the mitochondrial structure caused by myocardial ischemia, restored ATP production, and inhibited Ca2+ accumulation. Furthermore, electroacupuncture at Biao and Ben inhibited calcium accumulation and restored P2X7 receptor expression levels. The protective effect of the combination of Biao and Ben acupoints on mitochondrial function is that they restore P2X7 receptor expression, which regulates mitochondrial metabolism.