miRNA-491-5p Inhibited Cell Proliferation in Human Hepatocellular Carcinoma Through Phosphatidylinositol 3-Kinase/Protein Kinase B Signaling Pathway

Abstract

miRNA-491-5p was a short, noncoding RNA, usually down-expressed in various human tumors and regulated biological functions. However, the connection between miRNA-491-5p and hepatocellular carcinoma (HCC) remained unclear. Therefore, the role of miRNA-491-5p played in HCC has been detected in this research. The results indicate that miRNA-491-5p is obviously diminished in tissues and cells of HCC (P < 0.001; P < 0.05). After miRNA-491-5p over-expressing, cell multiplication as well as invasion viability were significantly inhibited (P < 0.01). Double luciferase reporter gene detection system demonstrated epidermal growth factor receptor (EGFR) gene was considered directly target miR-491-5p, along with suppressed EGFR was observed in cells over-expressed miRNA-491-5p. Moreover, miRNA-491-5p functioned via the PI3K/Akt signaling pathway. Generally, this study illustrated that miRNA-491-5p promoted HCC progression via PI3K/Akt signaling pathway targeting EGFR, while miRNA-491-5p mimicking therapeutics may provide viable avenue for the medication of HCC.