The Biological Effects of Forkhead Box Protein A2 (FOXA2) on Cervical Cancer Cells by Regulating Phosphatase and Tensin Homolog (PTEN)

Abstract

The pathogenesis of cervical cancer is complex and FOX family is abnormally expressed in several diseases. FOXA2’s role in cervical cancer remains unclear. FOXA2 level in cervical cancer and adjacent normal tissues was detected. Cervical cancer Hela cells were divided into control group, FOXA2 group and FOXA2 siRNA group followed by analysis of FOXA2 level by Real time PCR and western blot, cell survival by MTT assay, cell migration and invasion, and PTEN expression by western blot. The cells were divided into NC group, FOXA2 group and FOXA2+PTEN inhibitor group followed by analysis of cell behaviors by flow cytometry and PTEN expression by western blot. FOXA2 was significantly downregulated in cancer tissues compared with adjacent tissues (P <0.05) and associated with tumor size and FIGO stage (P <0.05), but not with vascular invasion, pathological grade and lymph node metastasis. Overexpression of FOXA2 inhibited Hela cell proliferation, migration and invasion, and increased PTEN expression (P <0.05), which were all significantly reversed after inhibition of FOXA2 (P <0.05). The addition of PTEN inhibitor to Hela cells overexpressing FOXA2 reversed the effect of FOXA2 on Hela cells and down-regulated PTEN expression (P <0.05). FOXA2 is downregulated in cervical cancer, which is related to tumor size and FIGO stage. Overexpression of FOXA2 inhibits cell behaviors by regulating PTEN.