The Downregulation of Long Noncoding RNA Metastasis Associated with Colon Cancer 1 (lncRNA MACC1-AS1) Inhibits the Migration and Invasion of Non-Small Cell Lung Cancer Cells
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Published:2021-03-01
Issue:3
Volume:11
Page:453-457
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ISSN:2157-9083
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Container-title:Journal of Biomaterials and Tissue Engineering
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language:en
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Short-container-title:j biomater tissue eng
Author:
Zhang Caidi1,
Zou Xinzhong1
Affiliation:
1. Department of Respiration, The Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214041, PR China
Abstract
To explore the effects of lncRNA MACC1-AS1 on the invasion and migration of non-small cell lung cancer (NSCLC), PCR was used to detect and compare lncRNA MACC1-AS1 expression in NSCLC cells to normal lung epithelial cells and assess the knockdown effect of lncRNA MACC1-AS1 in A549 and
H1299 cell lines. The Transwell system was employed to compare the invasiveness of the lncRNA MACC1-AS1 knockdown cells to the control cells. The migratory ability of the lncRNA MACC1-AS1 knockdown cells was compared to the control cells via in vitro scratch assay. Western blot was
used to detect the E-cadherin/N-cadherin expression. Balb/c mouse model was used to verify the metastasis ability in vivo between the control and lncRNA MACC1-AS1 knockdown groups. The level of lncRNA MACC1-AS1 was markedly higher in lung cancer cells than normal lung epithelial cells.
The knockdown of lncRNA MACC1-AS1 reduced the invasiveness and migratory capacity of A549 and H1299 cells. Downregulating lncRNA MACC1-AS1 upregulated E-cadherin but down-regulated N-cadherin in A549 and H1299 cells. Besides, the silencing of lncRNA MACC1-AS1 reduced the A549 cells’
ability to metastasize in vivo. LncRNA MACC1-AS1 can enhance the invasiveness and migratory capability of NSCLC.
Publisher
American Scientific Publishers
Subject
Biomedical Engineering,Medicine (miscellaneous),Bioengineering,Biotechnology