Research on the Expression and Regulatory Mechanism of Breast Cancer Susceptibility Gene-1 on Cell of Skin Cancer in Different Classification

Abstract

The expression condition of breast cancer susceptibility gene-1 (BRCA1) in tissue of skin cancer in different classification was assessed in our study with analysis of the correlation between BRCA1 and classification of skin cancer. The influence of BRCA1 on the tyrosine kinase receptors (c-kit) was detected to discuss the possible-existing regulatory mechanism. The skin cancer was classified according to the clinical symptoms and was divided into zero to the fifth phase. Then the expression of BRCA1 and c-kit in tissue of skin cancer in different classification was detected by RT-PCR. The cell line of skin cancer HS-4 cultured in vitro was transfected with high-BRCA1-expressed vector. There was negative correlation between the expression of BRCA1 and the classification of skin cancer. There was no significant difference among the zero phase, the first phase and the second phase when the value of P was less than 0.05. There was no significant difference between the third phase and the fourth phase when the value of P was larger than 0.05. But there was significant difference between the latter two groups and the former three groups when the value of P was less than 0.05. The expression of c-kit was increased along with the elevation of classification of skin cancer with significant difference among groups. The reducing of expression of BRCA1 could affect the expression of c-kit from the cell experiment. There was negative correlation between the expression of BRCA1 and the expression of c-kit. There was correlation between the expression of BRCA1 and c-kit and the development of skin cancer. There was negative correlation between BRCA1 expression and c-kit level. It was illustrated that the BRCA1 could develop certain regulatory effect on skin cancer by influencing the expression level of c-kit.