Affiliation:
1. Department of Obstet. & Gynecol., The First People’s Hospital of Wen Ling, Wenzhou Med. Univ., Affiliated Wenling Hosp., Wenling, Zhejiang, 317500, China
Abstract
The clinical prognosis of endometrial cancer is poor. We assessed the influence of IL-17 and FGF2 on HEC-1B cells to provide evidence for the clinical prevention and treatment. HEC-1B cells were assigned into control group, IL-17 group, FGF2 group, and IL-17+FGF2 group followed by analysis
of cell viability, IL-17 and FGF2 protein and mRNA content by Western Blot and RT-PCR. Under co-culture, the cell viability, migration and invasion and FGF2 level in IL-17 group, FGF2 group, and IL-17+FGF2 group were found to be significantly higher than those in control Group (P <0.05),
but the apoptosis rate was significantly lower than control group. The above changes were more significant in IL-17+ FGF2 group compared to other three groups (P < 0.05). IL-17 and FGF2 treatment in HEC-1B cells can significantly promote the viability, migration and invasion of malignant
tumor cells with more significant changes in the IL-17+FGF2 intervention group, suggesting these two have a synergistic effect. In conclusion, IL-17 and FGF2 exerts synergistic effect on promoting endometrial cancer cell migration and invasion, indicating that they might be novel targets for
the treatment of endometrial cancer.
Publisher
American Scientific Publishers
Subject
Biomedical Engineering,Medicine (miscellaneous),Bioengineering,Biotechnology