Preparation and Evaluation of the Curative Effect of Blue Shark (Prionace glauca) Skin Collagen Composite Gel in a Rat Oral Ulcers Model

Abstract

Objective: To evaluate the curative effect of blue shark skin collagen composite gel on oral mucosal ulcer using the rat oral ulcers model stimulated by glacial acetic acid. Methods: Collagen from blue shark skin was isolated and physiochemically characterized by FTIR, SDS-PAGE and scanning electron microscopy (SEM). Seventy standard male rats were divided into seven groups. The surface and the area of the ulcer were observed and calculated daily. After 12 days of administration, rats in the model group and the control group were killed and the ulcer and surrounding tissues were cut to pieces about one mm3 size. The specimens were stained with 10% formalin solution, paraffinembedded sections, HE staining and light microscope were used to observe the histopathological changes in ulcer tissues. Results: The high-dose group had the fastest ulcer healing effects after 12 days of treatment with blue shark skin collagen composite gel. The composite gel was found to significantly accelerate the healing of oral ulcers in a dose-dependent manner. Conclusion: The blue shark skin collagen composite gel in this study may be a good biomedical material candidate for the treatment of oral ulcers in the near future. Potential of other marine fish skin collagen comples on healing oral ulcers should be also considered.