microRNA-30e-5p Up-Regulation Subdues Osteosarcoma Cell Proliferation and Metastasis via Regulating Collagen Triple Helix Repeat Containing 1

Abstract

The cancerous and matched para-carcinoma tissues were gained from total of 40 patients with OS, miR-30e-5p level was monitored through qRT-PCR, and the relevance between abnormal expressed miR-30e-5p and clinical indicators was uncovered. Simultaneously, miR-30e-5p expressive level in common-applied OS cells was detected by qRT-PCR. Additionally, miR-30e-5p suppression and overexpression models were constructed, and CCK-8 and transwell procedures were subsequently executed to analyze the influences of miR-30e-5p in OS cells. Further, relation between miR-30e-5p and CTHRC1 was forecased, meanwhile CTHRC1 function in miR-30e-5p-modulated OS cells was explored. Low expressed miR-30e-5p is linked to the distant metastasis and poor prognosis, which may restrain the malignant progression of OS by targeting CTHRC1.