LncRNA Phosphoglucomutase 5-Antisense RNA 1 Modulation Affects the Proliferation and Metastasis Capabilities via Upregulating miR-484 Expression in Colorectal Cancer Cells

Abstract

Background: LncRNA emerges to be helpful in the diagnosis and treatment of colorectal cancer (CRC). We intended to uncover the underlying modulatory pathway of lncRNA PGM5-AS1 in CRC cells. Materials and Methods: The prediction of target of PGM5-AS1 was by informatics and verified by dual luciferase assay. Transcription levels of PGM5-AS1 and miR-484 were determined by qPCR. Cell viability was measured by cell counting kit (CCK)-8. Migration rate was assessed by wound healing assay and invasion by Transwell. Cell growth was evaluated by colony formation assay. Results: PGM5-AS1 was up-regulated in CRC tissue and cell lines while its down-regulation contributed to the decreasing of cell viability, growth, migration and invasion capabilities of SW480 and HCT116 cells. Moreover, miR-484 was the target of PGM5-AS1. In addition, the down-regulation of PGM5-AS1 partially restored the elevated cell viability, growth, migration and invasion capacities following the inhibition of miR-484 expression in SW480 and HCT116 cells. Conclusions: The loss of miR-484 expression in CRC might be involved in the promotion and metastasis of CRC, the consequence which could be the result of the overexpression of PGM5-AS1. Hence, the down-regulation of PGM5-AS1 could be a therapeutic target in the prevention or intervention of CRC.