Research on Autophagy of Gingiva Epithelial Cell Induced by Porphyromonas Gingivalis Regulated by Long Non-Coding RNA Transforming Growth Factor Beta-2 Overlapping Transcript 1 (LncRNA TGFB2-OT1)

Abstract

Our study analyzes lncRNA TGFB2-OT1’s effect on autophagy and migration of gingiva epithelial cells and porphyromonas gingivalis. The expression of TGFB2-OT1 and TIMP2 in GEC cells was detected by QRT-PCR. Cell proliferation and colon formation was assessed by MTT method and colony forming assay. The relation between TGFB2-OT1 and TIMP2 was confirmed luciferase assay. The autophagy, apoptosis rate and period distribution was detected by flow cytometry along with analysis of porphyromonas gingivalis of GEC cells. TGFB2-OT1 was upregulated significantly as shown by IncRNA array. TGFB2-OT1 overexpression in GEC cells improved cell proliferation and migration, enhanced the sensibility of gingiva epithelial cell on the porphyromonas gingivalis and increased autophagy. The GEC cells were blocked at G2/M period. The TIMP2 was a direct target of TGFB2-OT1. In conclusion, TIMP2 participates in the up-regulation of MMP10 induced by TGFB2-OT1. The lncRNA TGFB2-OT1 maybe be a potential biomarker to assess the autophagy of gingiva epithelial cell.