Upregulation of MicroRNA-199 (miR-199) Prompts the Apoptosis of K562 Cell in the Model of Mice with Leukemia

Abstract

We investigated miR-199’s effect on the apoptosis of leukemia cells (K562) as so to provide reference idea for a new therapeutic target. 20 normal and healthy BALB/c nude rats were selected and equally and randomly assigned into inoculated group and blank group. The K562 cell was obtained and then divided into blank control group, miR-199 mimic group, and miR-199NC group followed by analysis of miR-199 expression, cell activity and apoptosis as well as the expression of Bax, Bcl-2 and PCNA. Inoculated group showed significantly higher proportion of leukemia cells and myeloid cells than blank group. The expression of miR-199 (3.22±0.03) in miR-199 mimic group was significantly higher than other two groups (P < 0.05) without difference between other two groups (P > 0.05). Bax expression (1.16±0.10) in miR-199 mimic group was significantly higher, whereas Bcl-2 (0.02±0.01) and PCNA (0.47±0.05) expression was significantly lower than other two groups. Upregulation of miR-199 could restrain the expression of Bcl-2 and PCNA through upregulation of Bax, indicating that miR-199 might be a new therapeutic target.