Research on the Negatively Regulation of Long Intergenic Non-Coding RNA 00210 to miR-424-5p in Breast Cancer Cells

Abstract

In this work, we investigate the expression of long intergenic non-coding RNA 00210 (LINC00210) and its effects on the behavior of breast cancer cells. To this end, we measured LINC00210 and miR-424-5p expression using RT-qPCR. Bioinformatics, dual luciferase report experiments, and RT-qPCR were applied to determine the potential function of LINC00210 in the regulation of miR-424-5p. Four groups of T-47D cells were set up: si-NC, si-LINC00210, si-LINC00210 + anti-miR-NC, and si-LINC00210 + anti-miR-424-5p. Then, cell viability, apoptosis, migration, and invasion were detected, respectively. Western blot analysis was applied to measure the expression levels of E-cadherin, N-cadherin, Bax, and Bcl-2. Our results showed that breast cancer tissue highly expressed LINC00210 and slightly expressed miR-424-5p, and that a direct binding function of LINC00210 to miR-424-5p existed. Furthermore, many of the behaviors of T-47D cells in the si-LINC00210 group were affected, including reductions in cell viability, migration and invasion abilities, as well as decreased expressions of LINC00210, Ki67, Bcl-2, and N-cadherin, an increased apoptosis rate, and increased expressions of miR-424-5p, E-cadherin, and Bax. In addition, in comparison with the si-LINC00210 + anti-miR-NC group, the cell behaviors of T-47D cells in the si-LINC00210 + anti-miR-424-5p group were affected, including increased cell viability, migration and invasion abilities, and expressions of Ki67, Bcl-2, and N-cadherin, but reductions in E-cadherin and Bax. The results demonstrated the inhibitory effects of LINC00210 on T-47D cells, as well as the negative regulation of LINC00210 on miR-424-5p, leading to cell apoptosis. The results imply the potential value of LINC00210 as a therapeutic target for breast cancer.