Effects of Transforming Growth Factor-/β 1 and Concentrated Growth Factor on Growth, Proliferation and Osteogenic Differentiation of Bone Marrow Stem Cells
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Published:2021-01-01
Issue:1
Volume:11
Page:185-193
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ISSN:2157-9083
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Container-title:Journal of Biomaterials and Tissue Engineering
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language:en
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Short-container-title:j biomater tissue eng
Author:
Yu Bohan1,
Li Qin1,
Wang Fang1
Affiliation:
1. Department of Periodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China
Abstract
Objective: We aimed to explore the effects of transforming growth factor-β1 (TGF-β1) and concentrated growth factor (CGF) on growth, proliferation and osteogenic differentiation of bone marrow stem cells (BMSCs). Methods: CGF was extracted from
rat venous blood. BMSCs was isolated from rats. TGF-β1 or CGF was used to induced BMSCs to observe their effects on BMSCs. The expression levels of BMSCs surface antigens were detected by flow cytometry. ELISA was performed to test the expression of VEGF, TGF-β1, PDGF
and SDF-1 in BMSCs. qRT-PCR was carried out to test the expression of COL-I, OPN, OC and ALP in BMSCs. Transwell assay was used to detect the migration rates of BMSCs. The cell morphology of BMSCs was detected by Alizarin red staining. Results: BMSCs were successfully isolated from
rats. CGF could significantly increase the expression of TGF-β1, PDGF and VEGF in BMSCs. In addition, TGF-β1 and CGF notably increased the proliferation of BMSCs. And they not only improved the activity of ALD, but also increased the levels of ALP, OPN, COL-I and OCN
in BMSCs to prompt the growth of BMSCs. Conclusion: CGF combined with TGF-β1 could prompt the growth, proliferation and osteogenic differentiation of BMSCs.
Publisher
American Scientific Publishers
Subject
Biomedical Engineering,Medicine (miscellaneous),Bioengineering,Biotechnology