Study on Signal Transduction Pathway of Migration Inhibitory Factor in Angiogenesis of Cervical Cancer

Abstract

Cervical cancer is a common female malignancy worldwide that accounts for the second in women malignant tumor. Macrophage migration inhibitory factor (MIF) is an important tumor promoter that plays an important role in tumor invasion and metastasis. This study explored MIF and PD98059’s effect on cervical cancer Siha cells to analyze MIF impact on VEGF expression, so as to discuss whether MIF may influence cervical cancer angiogenesis through ERK/MAPK signaling pathway. MIF and PD98059 were adopted to treat Siha cells. Western blot were done to measure MIF, ERK1, and VEGF expression. MTT was applied to determine cell proliferation. ERK1 and VEGF were positively expressed in cells. Cell proliferation was elevated gradually at 24, 48, and 72 h in MIF group and declined in PD98059 group (P < 0.05). ERK1 and VEGF protein significantly elevated after treated by MIF for 48 hours and reduced after intervened by PD98059 for 48 h (P <0.05). ERK1 and VEGF obviously downregulated in both MIF and PD98059 treatment groups without difference between two groups (P <0.05). MIF, ERK1, and VEGF is expressed in cervical cancer SiHa cells. MIF promotes cell proliferation through regulating ERK/MAPK signaling pathway to upregulate ERK1 and VEGF expression.