Identification of the Role of Apoptosis-Related Genes in Myocardial Ischemia-Reperfusion Injury

Abstract

Myocardial ischemia-reperfusion injury (MIRI) is a complex phenomenon that often occurs in patients with ischemic heart disease. The potential molecular mechanism of MIRI needs to be more precise. This paper aims to identify the potential biomarkers of MIRI through a series of bioinformatics methods. As a kind of programmed cell death, focal death is closely related to inflammation. It plays a crucial role in tumor diseases. Therefore, this paper corrected several sets of transcriptome data of MIRI in the GEO cohort in batches, and differentially expressed genes (DEGs) were obtained. In addition, the genes related to scorch death were collected and intersected with DEGs to get the intersection gene. Then, we screen hub genes from the intersection genes based on three algorithms of cytoHubba. The hub genes were analyzed by the mRNA-miRNA interaction network, mRNA-TF interaction network, and mRNA-drug network. The hub genes obtained in this paper have interaction with many drugs, which may be the potential therapeutic target of MIRI. In addition, we performed RT-qPCR to validate the mRNA expression levels of hub genes.