Correlation Between Genotypes and Allele Frequency of Lymphotoxin-Alpha and Gastric Cancer via Magnetic Separation Dual-Color Fluorescent Genotyping

Abstract

Gastric cancer (GC), as fifth most prevalent cancer in the world, poses a serious threat to people's health, especially in China and some undeveloped countries. If early warning or susceptible population screening for gastric cancer can be achieved, its morbidity and mortality will undoubtedly be reduced. In this study, mutation of Lymphotoxin-alpha (LTA) gene rs909253 locus of 200 gastric cancer samples and 134 normal samples was completely detected via magnetic separation dual-color fluorescent genotyping method (MSFG), to analyze the correlation between LTA and GC. Results showed that there were 28 wild types, 48 mutant types, 57 heterozygotes and 1 failed genotyped normal sample. For cases, 194 samples were genotyped successfully, in which 14 samples were wild types, 75 samples were mutant types and other 105 samples were heterozygotes. Further statistical analysis found that mutant-genotype GG (OR (95%CI), 3.68 (1.80–7.55); P < 0 001) and heterozygous-genotype GA (OR (95%CI), 3.13 (1.50–6.53); P < 0 01) had significant correlation with gastric cancer, as well as allele-G frequency (OR (95%CI), 1.78 (1.27–2.48); P < 0 001) in subsequent analysis. These results suggest that the LTA gene is closely related to gastric cancer, and its mutation at rs909253 locus could increase the risk for gastric cancer. Detection of LTA gene mutation for screening gastric cancer susceptible population has therefore significant potential application value in clinic.