Facile Comparison of Nonspecific Adsorptions of Proteins on Magnetic Submicron Particles with Alkaline Phosphatases as the Probes
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Published:2019-11-01
Issue:11
Volume:11
Page:1574-1581
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ISSN:1941-4900
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Container-title:Nanoscience and Nanotechnology Letters
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language:en
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Short-container-title:nanosci nanotechnol lett
Author:
Xie Yanling,Chong Huimin,Li Yaping,Feng Yiran,Hu Xiaolei,Long Gaobo,Xie Wanjun,Yang Xiaolan,Liao Fei
Abstract
The soluble form of Escherichia coli alkaline phosphatase (ECAP), commercialized calf intestinal alkaline phosphatase (CIAP) and biotinylated CIAP (Biotin-AP) were tested as probes for nonspecific adsorptions of proteins on micro/nano materials represented by magnetic submicron
particles (MSP). The nonspecific adsorptions of three candidate probes were evaluated by directly measuring the activities of the adsorbed probes with 4-nitrophenylphosphate. The activities of those three candidate probes exhibited reasonable resistance to detergents, but the presences of
> 0.1% glycerol in the adsorption systems greatly reduced their nonspecific adsorptions on MSPs. The nonspecific adsorptions of ECAP and Biotin-AP on some MSPs were significant and saturable but became negligible after such MSPs were coated with small zwitterions, bovine serum albumin and/or
streptavidin. Only MSPs applicable to chemiluminescence immunoassays showed negligible nonspecific adsorptions of Biotin-AP and ECAP, but all of the tested MSPs exhibited negligible nonspecific adsorptions of CIAP. Therefore, Biotin-AP and ECAP were suitable probes for nonspecific adsorptions
of proteins on micro/nano materials.
Publisher
American Scientific Publishers
Subject
General Materials Science
Cited by
1 articles.
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