Effects of Macrophage Capping Protein (CAPG) on Glioma Cell Proliferation

Abstract

Malignant glioma is one of the most common primary brain tumors and is among the deadliest of human cancers. In recent years, detection of related genes has provided a new perspective for early diagnosis and treatment of glioma. Previous studies have shown that macrophage capping protein (gelsolin-like, CAPG) is highly expressed in tumor cells, but there have been very few studies on glioma cells. In our study, several candidate genes were identified by lentivirus-mediated RNAi technology, and the infected glioma U251 cells were detected by qRT-PCR using Cellomics Highly Intensive Functional Screening (HCS) platform. The results showed that after knockdown of CAPG, the expression of CAPG gene in U251 glioma cells decreased, cell cycle was blocked, the number of U251 cells decreased, and the apoptotic rate increased. These results suggested that CAPG is significantly associated with the development of glioma cells. Shortening of CAPG inhibited the proliferation of glioma cells. CAPG could be used as an important reference for predicting the occurrence and development of glioma, and it could also serve as a novel target of targeted therapy for glioma.