Affiliation:
1. Department of Orthopedic, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510260, China
Abstract
miR-218 is associated with osteogenesis. Bioinformatics analysis showed a targeting relationship between miR-218 and GSK-3β 3′-UTR. Our study assessed whether miR-218 regulates GSK-3β expression and affects osteoblast differentiation of bone marrow mesenchymal
stem cells (BMSCs). Osteogenic induction medium was used to induce BMSCs to differentiate into osteoblasts. miR-218, GSK-3β, β-catenin and RUNX2 level was detected during D10 and D20 differentiation. BMSCs cells were divided into antagomir-NC and antagomir-218, and
induced to differentiate for 20 days followed by analysis of GSK-3β, β-catenin and RUNX2 level, osteogenesis and cell differentiation by the alizarin red staining. Compared with pre-differentiation,the expression of miR-218, β-catenin and RUNX2 was gradually
increased and GSK-3β expression was decreased during the differentiation of BMSCs into osteoblasts. There was a targeted regulation relationship between miR-218 and GSK-3β. Compared with the antagomir-NC group, GSK-3β protein expression was increased in
antagomir-218 transfection group, with decreased the expression of β-catenin and RUNX2 protein, reduced ALP activity as well as weakened staining degree of alizarin red. GSK-3β expression is decreased and miR-218 expression is increased during osteoblast differentiation
of BMSCs. Inhibition of miR-218 expression can up-regulate GSK-3β expression and attenuate the ability of BMSCs to differentiate into osteoblasts.
Publisher
American Scientific Publishers
Subject
Biomedical Engineering,Medicine (miscellaneous),Bioengineering,Biotechnology
Cited by
1 articles.
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