Solute Carrier Family 5 Member 8 Inhibits the Proliferation and Migration and Promotes Apoptosis of Cervical Cancer Cells by Upregulating CCAAT/Enhancer Binding Protein Delta

Abstract

SLC5A8 has been studied in a variety of cancers as an anti-tumor factor. SLC5A8 is down-regulated in cervical cancer (CC), but its specific role in CC has not been reported. The expression of SLC5A8 and CEBPD was detected by RT-qPCR and western blot. Subsequently, cell viability was detected by CCK-8 technology and cell proliferation level was detected by cloning formation assay. The ability of CC cells was detected by Transwell assay.Wound healing detected cell migration. Cell apoptosis was detected by flow cytometry. The expression of apoptosis-related proteins bcl-2, Bax, caspase3 and caspase9 were detected by western blot. In addition, the immunoprecipitation technique detected the association between SLC5A8 and CEBPD. The results showed that the expression of SLC5A8 in CC cell lines was significantly decreased. After overexpression of SLC5A8, we found that the proliferation level of Hela increased, cell survival rate, invasion and migration ability decreased, and cell apoptosis increased. The expression of CEBPD in CC cells is also decreased. In addition, we found that SLC5A8 has a targeted regulatory effect on CEBPD. It interfered with the expression of CEBPD and inhibited the effect of overexpression of SLC5A8 on the proliferation, invasion, migration and apoptosis of Hela in CC. In conclusion, overexpression of SLC5A8 inhibited the proliferation, migration of CC cells and promoted apoptosis by upregulating the expression of CEBPD.