Mechanism of Polycaprolactone-Polyetherimide and pEGFP Nano-Composite and Bcl-2 Expression on Regulating Proliferation and Apoptosis of Cervical Carcinoma Cells

Abstract

This study assessed mechanism of Polycaprolactone (PCL)-Polyetherimide (PEI) PCL-PEI and Enhanced green fluorescent protein plasmid (pEGFP) nano-composite on cervical carcinoma cells and associated Bcl-2 expression. The co-polymer of PCL-PEI was synthesized and pEGFP nano-composite was prepared. The PCL-PEI+pEGFP and empty carrier pEGFP C1 were transfected into Hela cells, which were divided into PCL-PEI group, CI (PCL-PEI+pEGFP and empty carrier pEGFP C1) group and NC group (normoal conctrast group). Proliferative condition of HeLa cells was detected with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method, while invasive condition was detected with Transwell closet method. The cellular morphology was tested with Hoechst 33342 staining method and apoptotic condition was detected along with measurement of Bcl-2 and Bax. Results showed that, the quantity of GFP (green fluorescent protein) in the PCRL-PET Polycaprolactone (PCL)-Polyetherimide (PEI) (PCL-PEI) group was more than in NC group, and proliferative activity in PCL-PEI group was notably less than in the NC group. Moreover, no difference in proliferative activity was observed between NC and CI groups, and quantity of cross-membrane cells in PCL-PEI group was less than NC group without difference in the quantity of cross-membrane cells between NC group and CI group, and cell nucleus was intact in the NC and CI groups, with uniform IF color distribution and lack of apoptotic phenomenon. There were shrinking cells in the PCL-PEI group, and proliferation and apoptosis were restrained in the cervical carcinoma cells, which was prompted with PCL-PEI and pEGFP nano-composite. Finally, the expression of Bcl-2 in the cervical carcinoma cells was also restrained.